本產(chǎn)品僅用于科研，不用于臨床

英文名稱 Anti-phospho-RPS6KB1（Ser421）
中文名稱 磷酸化核糖體S6蛋白激酶抗體
別 名 RPS6KB1 （phospho S421）; RPS6KB1 （phospho Ser421）; p-RPS6KB1 （Ser421）; RPS6KB1; PS6K; S6K; S6K1; STK14A; p70（S6K）-alpha; p70-S6K; p70-alpha; ribosomal protein S6 kinase; 70kDa; p70 S6 Kinase; P70 Beta1; KS6B1_HUMAN; Ribosomal protein S6 kinase beta-1; S6K-beta-1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子
蛋白分子量 predicted molecular weight: 57kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Ser421
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR （mammalian target of rapamycin） signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013].
Function : Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 （mTORC1） leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase （EEF2K）, which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin.
Subunit : Interacts with PPP1R9A/neurabin-1. Interacts with RPTOR. Interacts with IRS1. Interacts with EIF3B and EIF3C. Interacts with POLDIP3 and TRAF4.
Subcellular Location : Cell junction, synapse, synaptosome.Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion. Isoform Alpha I: Nucleus. Cytoplasm. Isoform Alpha II: Cytoplasm.
Tissue Specificity : Widely expressed.
Post-translational modifications : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Similarity : Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism （By similarity）. Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
Database links : UniProtKB/Swiss-Prot: P23443.2
宿主：Human、Murine、Rat （Rattus）、Bovine、Dog （Canine）等物種
磷酸化核糖體S6蛋白激酶抗體應(yīng)用范圍: WB, ELISA, IP, IHC （p）, IHC （f）, IF
緩沖液：0.01m PBS,pH值7.4和10毫克/毫升BSA
儲(chǔ)存液：0.1%（w/v）*                                                         

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*mt 線粒體DNA拓普西異構(gòu)酶Ⅰ抗體Y55556 0.1ml
TOP2 alpha DNA拓普西異構(gòu)酶ⅡA抗體Y55557 0.1ml
TOPO II/TOPO II Alpha DNA拓普西異構(gòu)酶Ⅱ抗體Y55558 0.1ml
phospho-TOPO II Alpha （Ser1106） 磷酸化DNA拓普西異構(gòu)酶Ⅱ抗體Y55559 0.1ml
TPH/Trptophan Hydroxylase 色氨酸羥化酶抗體Y55560 0.2ml
phospho-TPH（Ser260） 磷酸化色氨酸羥化酶抗體Y55561 0.1ml
phospho-TPH（Ser19） 磷酸化色氨酸羥化酶抗體Y55562 0.1ml
TPO 甲狀腺過(guò)氧化物酶抗體Y55563 0.2ml
TRA16 睪丸激素受體相關(guān)蛋白16抗體Y55564 0.1ml
TRA16 睪丸激素孤兒受體相關(guān)蛋白抗體Y55565 0.1ml
TRADD 腫瘤壞死因子受體1相關(guān)死亡域蛋白抗體Y55566 0.1ml
phospho-TRADD（Ser299） 磷酸化腫瘤壞死因子受體1相關(guān)死亡域蛋白抗體Y55567 0.1ml
phospho-TRADD（Ser269） 磷酸化腫瘤壞死因子受體1相關(guān)死亡域蛋白抗體Y55568 0.1ml
phospho-TRADD（Ser274） 磷酸化腫瘤壞死因子受體1相關(guān)死亡域蛋白抗體Y55569 0.1ml
TRAF1 腫瘤壞死因子受體相關(guān)因子1抗體Y55570 0.2ml
TNFR2/TRAF2/CD120b 腫瘤壞死因子受體相關(guān)因子2抗體Y55571 0.1ml
TRAF3/CD40bp 腫瘤壞死因子受體相關(guān)蛋白3抗體Y55572 0.1ml
TRAF4/CART 1 腫瘤壞死因子受體相關(guān)蛋白4抗體Y55573 0.1ml
TRAF6 腫瘤壞死因子受體相關(guān)因子6抗體Y55574 0.1ml
TRAIL 腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體抗體Y55575 0.1ml
Transthyretin 轉(zhuǎn)甲狀腺素蛋白抗體Y55576 0.1ml
Transaldolase 1 轉(zhuǎn)醛醇酶抗體Y55577 0.2ml
Triosephosphate isomerase 磷酸丙糖異構(gòu)酶抗體Y55578 0.2ml
TRBF1/TRF1 端粒體復(fù)制結(jié)合因子1抗體Y55579 0.1ml
TREM1 髓系細(xì)胞觸發(fā)受體1抗體Y55580 0.2ml
TREM2 髓系細(xì)胞觸發(fā)受體2抗體Y55581 0.1ml
TREML1/TLT1 髓系細(xì)胞觸發(fā)受體樣轉(zhuǎn)錄因子1抗體Y55582 0.2ml
TREML2/TLT2 髓系細(xì)胞觸發(fā)受體樣轉(zhuǎn)錄因子2抗體Y55583 0.2ml
Ferritin 鐵蛋白抗體Y55584 0.2ml
Transferrin 轉(zhuǎn)鐵蛋白抗體Y55585 0.1ml
Transferrin（1F10） 轉(zhuǎn)鐵蛋白單克隆抗體Y55586 0.1ml
TRF2 端粒結(jié)合蛋白2抗體Y55587 0.1ml
omerase Binding Protein, P23 端粒酶結(jié)合蛋白P23抗體Y55588 0.1ml
phospho-p23 （Ser113） 磷酸化端粒酶結(jié)合蛋白P23抗體Y55589 0.1ml
TRIM32 TRIM32抗體Y55590 0.1ml
TrK A/NTRK1 酪氨酸激酶A抗體Y55591 0.1ml
Phospho-TrkA （Tyr490）/TrkB （Tyr516） 磷酸化酪氨酸激酶A抗體Y55592 0.1ml
Phospho-TrkA （Tyr674/675）/TrkB （Tyr706/707） 磷酸化酪氨酸激酶A抗體Y55593 0.1ml
Phospho-TrkA （Tyr785）/TrkB （Tyr816） 磷酸化酪氨酸激酶A抗體Y55594 0.1ml
Trk A/B/C 酪氨酸激酶A/B/C抗體Y55595 0.1ml
Trk B/NTRK2 酪氨酸激酶B抗體Y55596 0.1ml
Trk B/NTRK2 酪氨酸激酶B抗體Y55597 0.1ml
phospho-NTRK2/TrkB （Tyr515） 磷酸化酪氨酸激酶B抗體Y55598 0.1ml
phospho-TrkB （Tyr705） 磷酸化酪氨酸激酶B抗體Y55599 0.1ml
Trk C 酪氨酸激酶C抗體（神經(jīng)生長(zhǎng)因子受體的一種）Y55600 0.1ml
Trop-2 原肌球蛋白抗體Y55601 0.1ml
TRP2 酪氨酸酶相關(guān)蛋白2抗體Y55602 0.1ml
TRPC6 瞬時(shí)受體電位離子通道蛋白6抗體Y55603 0.1ml
Thioredoxin 硫氧還蛋白抗體磷酸化核糖體S6蛋白激酶抗體Y55604 0.1ml